dna clean and concentration kit Search Results


dna clean concentrator kit  (Zymo Research)
99
Zymo Research dna clean concentrator kit
Dna Clean Concentrator Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/pmc13076867-604-8-13?v=Zymo+Research
Average 99 stars, based on 1 article reviews
dna clean concentrator kit - by Bioz Stars, 2026-08
99/100 stars
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nucleospin gdna clean  (MACHEREY NAGEL)
96
MACHEREY NAGEL nucleospin gdna clean
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Nucleospin Gdna Clean, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/pmc07406498-278-9-13?v=MACHEREY+NAGEL
Average 96 stars, based on 1 article reviews
nucleospin gdna clean - by Bioz Stars, 2026-08
96/100 stars
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nucleospinrna clean up xs kit  (MACHEREY NAGEL)
91
MACHEREY NAGEL nucleospinrna clean up xs kit
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Nucleospinrna Clean Up Xs Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/pmc04084800-354-13-16?v=MACHEREY+NAGEL
Average 91 stars, based on 1 article reviews
nucleospinrna clean up xs kit - by Bioz Stars, 2026-08
91/100 stars
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double sided cleanup  (Zymo Research)
94
Zymo Research double sided cleanup
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Double Sided Cleanup, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/bio_rxiv__64898__2026__04__18__719392-153-39-41?v=Zymo+Research
Average 94 stars, based on 1 article reviews
double sided cleanup - by Bioz Stars, 2026-08
94/100 stars
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miniprep kit  (Zymo Research)
99
Zymo Research miniprep kit
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Miniprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/bio_rxiv__2023__01__27__525415-103-6-9?v=Zymo+Research
Average 99 stars, based on 1 article reviews
miniprep kit - by Bioz Stars, 2026-08
99/100 stars
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spineasy pcr purification  (Valiant Co Ltd)
92
Valiant Co Ltd spineasy pcr purification
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Spineasy Pcr Purification, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/pmc11013279-83-100-107?v=Valiant+Co+Ltd
Average 92 stars, based on 1 article reviews
spineasy pcr purification - by Bioz Stars, 2026-08
92/100 stars
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glycerol  (Chem Impex International)
95
Chem Impex International glycerol
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Glycerol, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/pmc07842297-62-133-134?v=Chem+Impex+International
Average 95 stars, based on 1 article reviews
glycerol - by Bioz Stars, 2026-08
95/100 stars
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zymo dna clean concentrator-5 kit  (Nordic BioSite)
90
Nordic BioSite zymo dna clean concentrator-5 kit
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Zymo Dna Clean Concentrator 5 Kit, supplied by Nordic BioSite, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/pmc09252859__jmedgenet___2021___107747supp001-11-14-16?v=Nordic+BioSite
Average 90 stars, based on 1 article reviews
zymo dna clean concentrator-5 kit - by Bioz Stars, 2026-08
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genomic dna clean & concentrator kit  (Lucerna-Chem ag)
90
Lucerna-Chem ag genomic dna clean & concentrator kit
Annotation of the loci found in the HDC island .
Genomic Dna Clean & Concentrator Kit, supplied by Lucerna-Chem ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/pmc05313534-106-20-26?v=Lucerna-Chem+ag
Average 90 stars, based on 1 article reviews
genomic dna clean & concentrator kit - by Bioz Stars, 2026-08
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gel/pcr dna fragment extraction kit promega-wizard sv gel and pcr clean up system kit  (Promega)
90
Promega gel/pcr dna fragment extraction kit promega-wizard sv gel and pcr clean up system kit
Annotation of the loci found in the HDC island .
Gel/Pcr Dna Fragment Extraction Kit Promega Wizard Sv Gel And Pcr Clean Up System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
gel/pcr dna fragment extraction kit promega-wizard sv gel and pcr clean up system kit - by Bioz Stars, 2026-08
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dna clean & concentrator kit  (Promega)
90
Promega dna clean & concentrator kit
Annotation of the loci found in the HDC island .
Dna Clean & Concentrator Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/pmc05204310-73-1-6?v=Promega
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dna clean & concentrator kit - by Bioz Stars, 2026-08
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dna clean up protocol from the promega wizard sv gel and pcr clean-up kit  (Promega)
90
Promega dna clean up protocol from the promega wizard sv gel and pcr clean-up kit
Annotation of the loci found in the HDC island .
Dna Clean Up Protocol From The Promega Wizard Sv Gel And Pcr Clean Up Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+and+concentration+kit/pmc09438399-315-7-13?v=Promega
Average 90 stars, based on 1 article reviews
dna clean up protocol from the promega wizard sv gel and pcr clean-up kit - by Bioz Stars, 2026-08
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Image Search Results


a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The genomic DNA used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .

Journal: Communications Biology

Article Title: Improvement of CRISPR/Cas9 system by transfecting Cas9-expressing Plasmodium berghei with linear donor template

doi: 10.1038/s42003-020-01138-2

Figure Lengend Snippet: a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The genomic DNA used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .

Article Snippet: The obtained genomic DNA was further purified using the Nucleospin gDNA Clean-up kit (Macherey-Nagel).

Techniques: Transfection, Plasmid Preparation, Mutagenesis, Hybridization, Purification, Cloning, Sequencing, Transgenic Assay

Annotation of the loci found in the HDC island .

Journal: Frontiers in Microbiology

Article Title: The Histidine Decarboxylase Gene Cluster of Lactobacillus parabuchneri Was Gained by Horizontal Gene Transfer and Is Mobile within the Species

doi: 10.3389/fmicb.2017.00218

Figure Lengend Snippet: Annotation of the loci found in the HDC island .

Article Snippet: DNA was extracted from L. parabuchneri with ZR Fungal/Bacterial DNA MiniPrep Kit (Lucerna Chem AG, Lucerne; Switzerland) and concentrated with Genomic DNA Clean & Concentrator Kit (Lucerna Chem AG) according to the manufacturer's instructions.

Techniques: Sequencing, Modification, Histone Deacetylase Assay